61st ICASS Short Courses

June 19-23, 2017

Confirmed workshops/short courses

1. Image Analysis (Jesse Greener, Université Laval, QC) – complimentary with registration

Increasingly, analytical sciences are focused on generating information-dense data sets to maximise the impact of research endeavours. High-resolution, quantitative, spatially resolved data is readily acquired by modern instrumentation, which ranges from standard microscopy to spectral imaging systems. Proper management, analysis and representation of these data sets are critical to their efficient use. In this workshop, Professor Jesse Greener will lead an image analysis workshop using powerful open-access image analysis software, ImageJ. Participants will gain hands on experience to analyse sample data sets, which include 2D, 3D images and videos. Various concepts will be covered related to: (i) setting up your workspace environment, (ii) image display enhancements and (iii) image manipulation and analysis, and (iv) special topics. Specifically, participants will learn:

(i) File management, manipulation of bit-depth, global image calibrations

(ii) Look up tables, image overlays, stacking images (for time series, z-stacks, ?-stacks)

(iii) Image calculations, intensity profiles, background correction for uneven lighting situations, and recording simple macros, and batch editing. In addition,

Participants are encouraged to bring their laptops preloaded with imageJ software which can be freely downloaded here . Example files will be made available to participants during the session.
This session will be held in the spirit of a laboratory class, where participants are expected to take notes and work on exercises provided. The time of the session will be announced shortly, and total length will be a half day.

2. Forensic Science and Reverse Engineering using Vibrational Spectroscopy (Sergey Shilov, Bruker Optics, USA) – complimentary with registration

Molecular spectroscopy (infrared and Raman) is among the most powerful tools in the forensic science and reverse-engineering process. Each molecule has a unique infrared and Raman signature, providing great specificity in the identification. The distribution of components can also be determined by collecting infrared and Raman images of the product in question. Vibrational spectroscopy will be presented as an important tool in determining the chemical composition of unknown materials and compounds with the goal of reducing or even eliminating the product development cycle. The following topics will be explored:

-Sample preparation techniques

-Product disassembly and analysis

-The role of FTIR and Raman microanalysis

-Chemical imaging and depth profiling

Examples will be shown demonstrating the chemical identification process using infrared and Raman spectroscopy, including a live demonstration. Attendees are encouraged to bring samples of interest for analysis. Please, e-mail to Jaclyn.Scanlon@bruker.com to register.

3. Inductively coupled plasma spectrometry (Diane Beauchemin, Queen’s University, Kingston, ON)($95 including taxes)

The inductively coupled plasma (ICP) is widely used as an emission source in optical emission spectrometry (OES) and as an ion source in mass spectrometry (MS). In its standard configuration, with a nebulization system, it enables multi-elemental analysis of solutions with a wide linear dynamic range. During this short course, the fundamental principles of the ICP will be briefly reviewed along with how to optimize the ICP operating conditions for robustness, i.e. freedom from matrix effects. The source of the remaining limitations will also be discussed along with simple strategies to alleviate them. Topics will include:

* Nebulization process

* Processes occurring in the ICP

* Selection of operating conditions and robustness indicator

* Calibration strategies

* Low-cost alternative sample introduction strategies

o Flow injection
o Vapour generation

The features of each approach will be highlighted using selected examples of applications.